BIO340 Recitation 5 Spring 2020

Your professor is having you look at two genes in Drosophila fruit flies to determine experimentally whether they are linked.  One trait is eye color and two alleles are present at the gene:  se⁺ is the wild-type allele for red eyes, and se is the mutant, recessive allele for sepia eyes.  The second trait is body color and two alleles are present at this gene:  e⁺ is the wild-type allele for grey body, and e is the mutant, recessive allele for ebony body.  You have crossed a pure-breeding red-eyed, grey body female by a pure-breeding sepia-eyed, ebony body male to generate F1 heterozygotes for a mapping cross. All the F₁ offspring have red eyes and grey bodies.  You next cross this F1 with pure-breeding sepia-eyed, ebony body flies and the following results were obtained.

165      red eyes, grey body

136      red eyes, ebony body

166      sepia eyes, ebony body

133      sepia eyes, grey body

A.  If the genes were linked, which two phenotypic classes would represent the recombinants?

B.  Conduct a Chi-Square analysis to determine if these two genes assort independently.

C.  If the genes do not assort independently, calculate the map distance between the two genes.

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